from Dr. Pollack -
10 February 2019 @ kl 23.59
Wow! So much information to digest. I’m afraid my answers might not satisfy.
Regarding your first bullet, I can say “yes.” Our studies have shown that both bubbles and droplets are enveloped by EZ shells. So, I would hazard a guess that any bubbles/droplets you see, either inside or outside the cell, contain these shells. The structure of these shells is described in detail in my recent book. Not sure you’ve seen “The Fourth Phase of Water” but it’s become popular. Whether is participates in tensegrity is another issue - the structure seems to differ from the icosahedron you mention - we see it as a hexagonal lattice, as argued in the above-mentioned book.
Regarding the second bullet, once again, we see the structure as layered, just like the structure around the bubble/droplet. My understanding is that the fascia consists mostly of connective tissue, but likely SURROUNDED by EZ water. On the other hand, the mechanical properties would appear to be some combination of the mechanical properties of the connective tissue AND the enveloping EZ water.
Hope that helps…. maybe a little?
to Dr. Pollack -
10 February 2019 @ kl18.51
Gerald H. Pollack, PhD
Professor of Bioengineering - Box 355061
University of Washington
Seattle, WA 98195
What is within and around a vacuole, space, little droplet? This is the reason I am writing to you, Dr. Pollack. You have a team behind you. And a curious mind.
Can you help me figure out the puzzle of HOW life fits together based on the foundation = the 4th Phase of Water?
Based on your speech at, EU2017: Future Science @29:51 “The dots or little droplets or little bubbles are the structure, lined by EZ material like an onion skin.”
• The structure of “little droplets or little bubbles” are the icosahedrons which provide biotensegrity
• “EZ material like onion skin” is the fascia which is a biofilm created by bacteria.
I write this based on the following information.
According to Bonnie Bassler at Princeton’s Bassler Labs, “bacteria build biofilms.” Her lab works on how bacteria communicate and how they control collective behaviors.
A physicist joined Bassler Labs in 2013, Knut Drescher, “I don’t know anything about biology but I know physics.” He studied a fluid flow dynamics.
How physics plays out in biology? Knut said, “One must move away from working in a flask, and mimic the world of the bacterium” @ 28:20…and that is a biofilm. He said, “The world works under pressure driven flow. It is more about curves than flat surfaces. Everything has curves, only in the lab, samples are grown in flat surfaces.”
The space within trabeculae of bone teams with its own ecosystem. Perhaps this is a form of biofilm. https://en.wikipedia.org/wiki/Trabecula
When crossing fluid-filled spaces, trabeculae may have the function of resisting tension (as in the penis, see for example trabeculae of corpora cavernosa and trabeculae of corpus spongiosum) or providing a cell filter (as in the trabecular meshwork of the eye).
A trabecula (plural trabeculae, from Latin for "small beam") is a small, often microscopic, tissue element in the form of a small beam, strut or rod that supports or anchors a framework of parts within a body or organ. A trabecula generally has a mechanical function, and is usually composed of dense collagenous tissue (such as the trabecula of the spleen). They can be composed of other materials such as muscle and bone.
Bone bleaching is, like the bleaching of Coral Reefs, called osteoporosis. Life is all around, but the bacterial biofilm cannot stick where it needs to stick. Bacterial life cannot exist for long in two and three dimensions without a biofilm. Therefore, there is loss of life. The bone dies and becomes brittle, despite the activity surrounding it.
Treat a bone with hydrochloric acid (Ca PO4)(OH2). The acid digests the mineral scaffolding aka biotensegrity structure. The collagen protein remains. Without the minerals, the bone is bendy, rubbery.
Treat a bone with bleach (hypochlorite). The bleach digests the collagen protein. The mineral scaffolding, the tensegrity structure, is left over. Without the collagen, the bone is brittle.
Could collagen actually be a microbial fiber? Currently it is thought to be inert, but at one time Schwann Cells were considered inert peripheral glial cells.
Science is full of Oops moments. R. Douglas Fields changed the “Thinking of the Day” in one experiment. (https://www.scientificamerican.com/article/the-other-half-of-the-bra/) R. Douglas Fields, a researcher at the National Institute of Health in the USA, proved in an experiment Schwann cells attach to long axons and form myelin around nerves. The “Thinking of the Day” was: Schwann cells were inert like the plastic coat around a wire cannot feel the electricity within the wire. Fields was imaging cultures and decided to put in Schwann Cells. Low and behold, the calcium change was detected in the surrounding neurons.
Ignaz Semmelweis in the mid 1800s asked his students to wash with chloride of lime (calcium hypochlorite) before they examined women in the hospital. The death rate from child bed fever aka sepsis declined. Bleach kills pathogenic bacteria. This good if the tissue has pathogenic sepsis. Possibly, bad if it kills commensal tissue bacteria as well.
Egyptian mummies’ bones have been found to contain a molecule safe to animal tissue, an antibiotic, tetracycline. Egyptians often drank beer bread. The process of fermenting the beer with bread, gave the beer an elixir of life quality. Basically, a low grade antibiotic which solved a lot of infectious problems at the time. The Scottish chemist Sir Alexander Fleming found something similar in 1921 a bacteriolytic substance, a lysozyme. Then in 1928, he serendipitously found an antibiotic, a mould, called penicillin.
Your information about Bound Water, Liquid Crystal Water, 4th Phase of Water, is thrilling, especially for Body Workers. This hydrophilic water reminds me of the fluid surrounding fascia and the proteoglycans of the ECM. It is the fluid within the scaffolding which contains nano microbial life. The Exclusion Zone, a vacuole? a biofilm?, “The dots or little droplets or little bubbles are the structure, lined by EZ material like an onion skin.”
Dr. Jean-Claude Guimberteau, The french hand surgeon which gave the world intimate views of the body, “Strolling Under the Skin”, said the space is called a vacuole. The vacuole is critical. The dew like fluid around it, holds up the pyramid, cell, icosahedron, per Dr Tom O’Byran Interconnected Series 9 Dec 2018.
Dr. George Tetz and others have done research on small life forms called bacteriophages. Bacteriophages are a type of virus that can infect bacteria and alter their function. First identified in 1917 by the former Soviet Union in their quest for something like an antibiotic.
Dr. George Tetz on Episode 77 of the Empowering Neurologist:
“So as for now, Bacteriophages are the most widely spread biological entities in the world. For example, one drop, just one drop of seawater, it contains 10 in 8th degree of different bacteriophages.
If we take a look into our microbiome, that and play a little bit with numbers, so we will get that the human body consists of 10 in 13 fold of eukaryotic cells. The number of bacteria associated with our microbiome is from 3 up to 5 times more. And the number of bacteriophages is 10 times more than the number of bacterial cells.
In other words the number of bacteriophages associated with our microbiota, it's eventually like 50 times more than human cells.”
Thank you for reading,
Characteristics of Bacterial Biofilms during Long-Term Culturing
Tetz, G V; Artemenko, N K; Zaslavskaya, N V; Tetz, V V.
Bulletin of Experimental Biology and Medicine; New York Vol. 155, Iss. 4, (Aug 2013): 467-9. DOI:10.1007/s10517-013-2180-7
“The Properties of Biofilms during their long-term existence are poorly understood.”